梓醇对3T3-L1脂肪细胞糖脂代谢的影响及其机制研究

Effect of Catalpol on Glucose and Lipid Metabolism in 3T3-L1 Adipocytes and Its Possible Mechanism

目的观察梓醇对3T3-L1脂肪细胞糖脂代谢及过氧化物酶体增长因子活化受体(PPAR-γ)蛋白表达的影响。方法采用3T3-L1前脂肪细胞诱导分化为成熟脂肪细胞,以葡萄糖氧化酶法检测细胞的葡萄糖消耗,以油红O染色法检测梓醇、罗格列酮对前脂肪细胞分化的影响,以Western Blot法检测各组PPAR-γ蛋白的表达。结果与空白对照组比较,梓醇组、罗格列酮组3T3-L1脂肪细胞的葡萄糖消耗量均明显增高(P<0.01),梓醇组与罗格列酮组比较差异无统计学意义(P>0.05);罗格列酮组OD值及PPAR-γ蛋白的表达较空白对照组明显升高(P<0.05);梓醇组OD值及PPAR-γ蛋白的表达较空白对照组明显降低(P<0.05)。结论梓醇在抑制PPAR-γ蛋白表达的同时能够促进葡萄糖的消耗,初步表明其具有体外调节脂肪细胞糖脂代谢的作用。

Objective To observe the effect of catalpol on the glucose and lipid metabolism and the protein expression of peroxisome-activated receptor （PPAR-γ） in 3T3-L1 adipocytes. Methods 3T3-L1 preadipocytes were induced to differentiate into mature adipocytes. The effects of eatalpol and rosiglitazone on preadipocytes differentiation were evalu- ated through the examination of glucose consumption by glucose oxidase method, observation of cell differentiation by oil red O staining, and detection of PPAR-7 protein expression by Western Blot analysis. Results Comparing with the blank control group, the glucose consumption in 3T3-L1 adipocytes of catalpol group and rosiglitazone group both significantly increased （P 〈 0.01 ）, but the differences between the observation groups were insiguificant（P 〉 0.05）. Rosiglitazone group had higher OD value and PPAR-γ protein expression than the blank control group（P 〈 0.05）. On the contrary, OD value and PPAR-γ protein expression of group were significantly decreased （P 〈0.05）. Conclusion Catalpol has inhibitive effect on the protein expression of PPAR-γ and can also promote the glucose consump- tion of adipocytes, which preliminarily indicates that catalpol would regulate glucose and lipid metabolism in adipocytes cells in vitro.