中药治疗肝纤维化腹膜淋巴孔调控及促腹水转归的研究

Study on the Regulatory Effect of Chinese Herbal Medicine on Peritoneal Lymphatic Stomata and in Enhancing Drainage of Ascites in Mice with Liver Fibrosis

目的 :应用CCl4 诱导的小鼠肝纤维化 ,观察两种中药复方对肝纤维化小鼠腹膜淋巴孔的调控作用和治疗腹水的意义。方法 :( 1)用CCl4 灌胃建立小鼠肝纤维化模型 ;( 2 )应用两种中药复方灌胃观察药物对肝纤维化的预防和治疗作用 ;( 3)以HE和VG染色 ,病理组织学观察 ;( 4 )扫描电镜和计算机图像处理 ;( 5)尿量及尿离子浓度检测。结果 :两种中药复方的预防组和治疗组肝组织病理变化轻 ,腹膜淋巴孔孔径增大 ,开放数量增多 ,分布密度高。尿量和尿Na+排出增多。其中中药复方Ⅰ组的作用优于中药复方Ⅱ组。结论 :两种中药复方均有明显减轻肝组织纤维化作用 ,并能调控腹膜淋巴孔 ,促进腹膜腔内液体转归 ,使尿量和尿Na+排出增加 ,减轻水钠潴留 。

Objective: To observe the regulatory effect of Chinese herbal medicine on peritoneal lymphatic stomata in carbon tetrachloride induced mouse model of liver fibrosis and its significance in treating ascites. Methods: (1) To form the mouse model of liver fibrosis by intragastric administration of carbon tetrachloride. (2) Two kinds of Chinese herbal compound prescriptions were given separately by gastrogavage to observe their effect on prevention and treatment of liver fibrosis. (3) Hematoxylin and eosin and van Gieson staining were used in the observation of pathology and histology. (4) Scanning electronmicroscope and computer image processing were used. (5) urinary volume and sodium ion concentration were measured. Results: The fibrotic change of the mouse models was alleviated with using both the prevention or treatment groups of the two compound prescriptions. The diameter of lymphatic stomata enlarged with increased number of opening and density of distribution. Urinary volume and sodium ion excretion increased after treatment. The effects displayed more significant in the group treated by compound prescription. Conclusion: Both kinds of Chinese herbal compound prescriptions have marked effect in alleviating liver fibrosis, regulating peritoneal lymphatic stomata, improving the drainage from the peritoneal cavity, causing increase of urinary volume and sodium ion excretion to reduce the water and sodium retention, and thus have favorable therapeutic effect in treating ascites.