经前期综合征肝气郁证大鼠下丘脑ERβ的表达及配体受体结合活性的研究

Study on Hypothalamal Estrogen Receptor Beta Expression and Ligand-receptor Binding Activity in Premenstrual Syndrome Model Rats with Liver-Qi Stagnation

目的探讨经前期综合征(PMS)肝气郁证发病的微观分子机理和中药经前舒颗粒对PMS肝气郁证的干预机制。方法雌性SD大鼠随机分为3组:正常对照组、模型组和经前舒组。慢性束缚应激法复制PMS肝气郁证大鼠模型,以经前舒颗粒进行药物干预。放射性配体受体结合技术测定下丘脑中雌激素受体β亚型(ERβ)的结合活性变化;Western blot技术检测下丘脑中ERβ蛋白的表达。结果与正常对照组比较,PMS肝气郁证模型组大鼠下丘脑中ERβ的配体受体结合活性、蛋白表达显著升高(P<0.05),而经前舒组可纠正其异常升高。结论下丘脑中ERβ表达量、配体受体结合活性的升高可能是导致PMS肝气郁证的重要病因;经前舒颗粒对下丘脑中ERβ表达、配体受体结合活性的异常变化有纠正作用。

Objective To investigate the molecular pathogenesis of premenstrual syndrome （PMS） with liver-Qi stagna tion, and to explore the therapeutic mechanism of Jingqianshu Granule for PMS with liver-Qi stagnation. Methods Female SD rats were randomly divided into three groups, i.e., the normal group, the modal group and the medication group. The PMS model rats with liver-Qi stagnation were induced by bondage stimulation. Jingqianshu Granule at the dose of 10 g·kg-1 was given to the rats in the medication group by intragastric administration during the modeling. Equal volume of sterilized water was given to the rats in the model group and the normal group, once daily. The ligand binding activity of estrogen receptorβ （ERβ） was detected by radio ligand-receptor binding techniques, and Western blot method was used to detect protein expression level of ERβ in rat hypothalamus. Results The protein level and estrogen-binding capacity of ER β in the hypothalamus were increased more obviously in the model group than those in the normal group （P 〈 0.05）, and the abnormal increase was corrected in the medication group. Conclusion The increase of hypothalamal ERI5 expression and its estrogen-binding capacity contributes to the development of PMS with liver-Qi stagnation, and Jingqianshu Granule has down-regulation of the hypothalamal ERβ expression and its estrogen-binding capacity.