祛风定痛方对小鼠偏头痛模型的影响

Effect of Qufeng Dingtong Recipe on Mice Migraine

目的探讨祛风定痛方对小鼠偏头痛模型的作用。方法复制利血平致低五羟色胺(5-HT)偏头痛模型,观察祛风定痛方对模型小鼠脑组织5-HT水平、凝血时间的影响及其止痛效果。结果祛风定痛方高、中、低剂量组与模型组比较均能明显升高利血平致低5-HT模型小鼠脑组织中5-HT水平(P<0.05),明显延长小鼠凝血时间(P<0.05,P<0.01),有效抑制福尔马林致小鼠疼痛反应,减少小鼠Ⅰ时相舔足时间(P<0.01),但不能够减少小鼠Ⅱ时相舔足时间;祛风定痛方能够提高电刺激致大鼠疼痛痛阈,其高剂量组在给药后30,60,90,120 min时与正常对照组比较,差异有统计学意义(P<0.01),中剂量组在给药后60,90 min时与正常对照组比较,差异有统计学意义(P<0.01,P<0.05),小剂量组在给药后60 min时与正常对照组比较,差异有统计学意义(P<0.05)。结论祛风定痛方治疗偏头痛可能与升高脑组织5-HT水平、延长偏头痛小鼠凝血时间有关,提示其有良好的镇痛作用。

Objective To investigate the effect of Qufeng Dingtong Recipe （QDR） on mice migraine. Methods The 5-hydroxytryptamine （5-HT） migraine mice models were induced by reserpine. After the modeling, we observed the effect of QDR on cerebral 5-HT level and clotting time of migraine mice, and also investigated its analgesic action. Results Large-, middle- and small-dose of QDR significantly increased cerebral 5-HT level （P 〈 0.05）, prolonged the clotting time （P 〈 0.05, P 〈 0.01）, relieved the pain induced by formalin, shortened mice licking paw time of phase I （P 〈 0.01 ）, but had no effect on the licking time of phase 1]. QDR increased the pain threshold induced by electric stimulation, and the effect of large-dose QDR group 30, 60, 90, 120 min after the medication differed from that in the blank control group（P 〈 0.01）. Middle-dose QDR increased the threshold of pain in 60,90 rain （P 〈 0.01, P 〈 0.05） and small-dose QDR increased the threshold of pain in 60 min compared with the blank control group （P 〈 0.05）. Conclusion The therapeutic mechanism of QDR for migraine is probably related with the increase of cerebral 5-HT level, prolongation of clotting time and enhancement of analgesic action.