二冬汤对大鼠尿液代谢产物的影响

Effect of Erdong Decoction on Urinary Metabolites of Rats

目的研究滋阴方二冬汤对大鼠内源性代谢产物的影响。方法取SD大鼠6只,灌胃给予二冬汤,分别于给药前1 d及给药5、10 d的末次给药后,收集4 h内的尿液,经过液相色谱分离,三重四极杆质谱检测尿液中内源性代谢物信息,采用主成分分析法降维、正交信号校正、偏最小二乘法判别分析法,分析二冬汤在不同给药天数时内源性代谢产物的差别,以及可能的生物标记物。结果给药5,10 d后引起变化的生物标记物,与给药前1 d比较,均有明显的变化。给药5 d的数据比较集中,给药10 d的数据比较分散。二冬汤给药5 d的样本点具有良好的聚类特征,提示给药5 d后二冬汤对大鼠尿液中内源性物质的代谢产生显著性影响,其中,内源性物质含量变化上升的有16个,下降的有17个。结论二冬汤给药后对大鼠内源性物质有影响,其生物学功能可能涉及到提高机体免疫力、促进机体的能量代谢、抗菌、抗病毒、抑制肿瘤细胞转移等,为二冬汤的开发和利用提供参考。

Objective To investigate the changes of endogenous metabolites concentrations in the urine of rats administered with Erdong Decoction. Methods The urine samples were collected one day before administration, and 5 and 10 days after administration of Erdong Decoction in rats. LC-MS/MS was used for the analysis of the urine, and then the acquired data were analyzed by principal component analysis （PCA）, orthogonal signal correction （OSC） and partial least square method （PLS） to find the difference of endogenous metabolites in different time and to catch the possible biological tag. Results The urinary endogenous metabolites concentrations on the fifth day and the 10th day of administration were obviously different from those one day before the administration. The data on the fifth day were rel- atively centralized, but the data on the tenth day were relatively scattered. The data of the fifth day had good clustering characteristics, indicating that Erdong Decoction had obvious effect on urinary endogenous metabolites 5 days after ad- ministration, and the changes were shown as the decrease in 16 biomarkers and the decrease in 17 biomarkers. Con- clusion The administration of Erdong Decoction shows an effect on the endogenous metabolites, and its biological function may be involved in enhancing immunity, promoting energy metabolism, counteracting bacteria and virus, and inhibiting the metastasis of tumor cells, which will benefit to the development and utilization of Erdong Decoction.