改良方法建立兔VX2肝癌模型及其MRI表现

Improved Methods of Establishing Rabbit VX2 Carcinoma Model and its MRI Findings

目的:利用经典和改良后的造模方法分别建立兔VX2肝癌模型并进行MRI扫描。方法:将40只新西兰大白兔随机分成两组,A组20只,使用经典的开腹瘤块注射法,B组20只,使用改良CT下定位瘤块注射法。分别于术后14、17、21、25d进行MRI扫描[1]。观察指标:(1)两种方法的造模成功率。(2)两种接种方法的操作时间的差别。(3)兔VX2肝癌模型在影像学中的表现。结果:(1)A、B两组的造模成功率分别为87.5%和83.3%,差异无统计学意义(P>0.05)。(2)A、B两组的平均接种时间分别为(28.6±4.90)min及(14.8±2.48)min,差异具有统计学意义(P<0.05)。(3)MRI扫描可以发现直径较小的肝肿瘤组织。结论:改良后的方法接种时间短、操作方便、成功率高,适用于建立稳定的兔VX2肝癌模型[2]。

Objective：Classic and improved modeling methods were established in rabbit VX2 carcinoma model using MRI scan. Methods：40 New Zealand white rabbits were randomly divided into two groups. The group A ot 20, with abdominal tumor mass, injected with classic method. Another group B of 20 with improved method. CT guided tumor mass injection. With 14,17,21,25 postoperative days MRI scanEl3. Indicators： （1）Success rate in two methods of modeling; （2） different operation time in two inoculation methods; （3） imaging of rabbit VX2 carcinoma model. Results：The modeling success rate of two groups A and I5 were 87.5% and 83. 3%, respectively;the differenee was not statistically significant （P〉0. 05）. The average vaccination time of group A and group B were （28. 6士4. 90）min and （14. 8 士 2. 48） rain respectively. A statistically significant difference （P〈0.05）. MRI scan are able to find liver tumor tissue even it is of small diameter. Conclusion： The improved method of inoculation time is short, easy to operate, high success rate, applied to establish a stable rabbit VX2 carcinoma modelEz3.