先天性胫骨假关节病变组织增生活性的研究

Immunohistochemical study of proliferating cell nuclear antigen in congenital tibia pseudarthrosis

目的 明确先天性胫骨假关节 (CPT)不同部位病变组织增生活性强弱和PCNA表达与年龄及复发的关系。方法 采用免疫组化方法 (SABC法 )对 2 1例CPT的三个不同取材部位所获得的 63个标本进行了PCNA检测。结果 CPT病变软组织的PCNA阳性表达率比创伤性假关节高 ,相差非常显著 (P <0 .0 1)。PCNA的阳性表达与年龄呈负相关 ,与复发呈正相关。结论 CPT病变组织具有较强细胞增生活性 。

Objective To study the proliferating activity in different areas of congenital tibia pseudarthrosis (CPT) and relationship of proliferating cell nuclear antigen (PCNA) expression with aging and relapsing. Methods PCNA was studied immunohistochemistry (SABC procedure) in 63 specimens from three different areas of 21 CPT patients. Results The positively expressed PCNA was significantly higher in the soft tissues in CPT than that in traumatic pseudarthrosis ( P < 0.01 ). The expression of PCNA had a parallel relation with the recurrence of CPT but a converse relation with the age of the patients. Conclusion The strong activity of cellular proliferation in the pathologic tissues of CPT theoretically supports the clinical findings: the lower the age is, the higher the recurrence.