大鼠拟血管性痴呆模型的建立及中药9602防治作用初探

Establishing of the Imitative Vascular Dementia Rat Model and the Preliminary Study on the Preventive and Treating Effects of TCM Preparation 9602 on the Disease

用反复夹闭双侧颈总动脉 (CCA)结合腹腔注射硝普钠将血压降低到 50mmHg左右的方法复制了大鼠拟血管性痴呆的模型。结果表明脑缺血再灌注后 7d ,行为学实验显示模型组大鼠出现明显的学习记忆障碍 ;形态学观察发现海马CA1区出现明显的细胞缺失 ,而CA3区无明显变化。中药 96 0 2可明显减轻脑缺血对海马的损伤 ,改善脑缺血引起的学习记忆障碍的作用。

The imitative vascular dementia rat model was established by repeatedly clipping the common carotid artery of the rat in combination with reducing the rat's blood pressure to about 50 mm Hg with an abdominal injection of sodium nitroprusside. On the 7th day after cerebral ischemic reperfusion, rats in the model group showed obvious disturbance in study and memory; and their hippocampal CA1 region manifested obvious cell deletion while there was no significant change in the CA3 region. The authors consider this kind of model reliable and reproducible, and suitable for studying the mechanism of the injury caused by cerebral ischemic reperfusion, and for observing the relevant drug effects. In the same experiment, it was found that TCM preparation 9602 could significantly relieve the injury of the hippocamp and the disturbance in study and memory caused by cerebral ischemia.