星状神经节阻滞对老龄大鼠术后认知功能的影响

Effect of stellate ganglion block on postoperative cognitive function in aged rats

目的评价星状神经节阻滞（SGB）对老龄大鼠术后认知功能的影响。方法健康雄性SD大鼠64只，18～20月龄，体重500～700g，采用随机数字表法，将其分为4组（n=16）：正常对照组（C组）、手术组（O组）、生理盐水＋手术组（NS组）和SGB＋手术组（SGB组）。SGB组注射0．25％布比卡因0．15ml行右侧SGB，NS组给予等容量生理盐水。给药结束后15min时O组、SGB组和NS组均实施剖腹探查术，手术时间30min。于术后1d每组取10只大鼠，进行水迷宫实验，连续6d，记录逃避潜伏期和穿越平台次数；分别于术后1、2、3d时取2只大鼠，取海马组织，HE染色，光镜下观察病理学结果。结果与c组比较，O组和NS组术后1～5d时逃避潜伏期延长，SGB组术后1、2d时逃避潜伏期延长，O组、NS组和SGB组穿越平台次数减少（P〈0．05）；与O组比较，NS组术后2～4d时逃避潜伏期缩短（P〈0．05）；与NS组比较，SGB组术后1～5d时逃避潜伏期缩短，穿越平台次数增加（P〈0．05）；SGB组L。时海马神经元数量明显多于NS组。结论SGB可改善老龄大鼠术后认知功能。

Objective . To investigate the effect of stellate ganglion block （SGB） on the postoperative cognitive function in aged rats. Methods Sixty-four male Sprague-Dawley rats, aged 18-20 months, weighing 500- 700 g,were randomly divided into 4 groups （n = 16 each） ： control group （group C）, operation group （group O）, normal saline ＋ operation group （group NS） and SGB ＋ operation group （group SGB ） . Group SGB received right SGB with 0.25% bupivacaine 0.15 ml, while the equal volume of normal saline was given in group NS. Groups O, SGB and NS underwent 30 rain of exploratory laparotomy starting from 15 min after the end of adminis- tration. Morris water-maze test was performed at days 1-6 after operation in 10 rats chosen from each group. The escape latency and frequency of crossing the original platform were recorded. Two rats were chosen from each group at 1, 2 and 3 d after operation and sacrificed and the hippocampi were removed for microscopic examination. Resuits Compared with group C, the escape latency wag significantly prolonged on 1-5 days after operation in groups O and NS, the escape latency wag significantly prolonged on 1 and 2 days after operation in group SGB, and the frequency of crossing the original platform was decreased in groups O, NS and SGB （ P 〈 0.05）. Compared with group NS, the escape latency wag significantly shortened, and the frequency of crossing the original platform was increased on 1-5 days after operation in group SGB （ P 〈 0.05） . The number of hippocampal neurons was significantly larger at 2 and 3 d after operation in group SGB than i.n group NS. Conclusion SGB can improve the postoperative cognitive function in aged rats.