不同啤酒生物胺含量的比较

The comparison of biogenic amine content in different beers

使用反相高效液相色谱法,测定啤酒中8种常见生物胺含量。以苯甲酰氯为衍生试剂,Agilent EclipseXDB-C18为固定相,乙腈和乙酸铵溶液为流动相,紫外检测波长设置为254 nm,梯度洗脱。本文首先对国内市售啤酒生物胺种类及含量范围进行研究,检测结果表明国内啤酒中8种生物胺均有存在,但不同样品所含种类不同,且有个别胺含量偏低无法定量。不同厂家的产品在种类和含量上存在差别,但整体含量接近,生物胺总量为4.21～10.59 mg/L,不足以对人类健康产生危害。原麦汁浓度、酒精度与生物胺含量的关系显示,二者与生物胺有一定联系,但非主要因素,相对于生产厂家和发酵情况,原麦汁浓度、酒精度对生物胺含量的影响较小。

In this paper,the reversed phase high performance liquid chromatography（HPLC） method was applied to detect eight kinds of common biogenic amine content in beers.It took the benzoyl chloride as derivative reagent,Agilent Eclipse XDB-C18 as stationary phase,acetonitrile and ammonium acetate solution as mobile phase.Ultraviolet detection wavelength of 254 nm and gradient elution were set.First of all,biogenic amine types and the range of its content were studied.Test results showed that the commercially available beers contained 8 kinds of biogenic amines,but different samples contained different types.And some amines content was too low to be quantified.The products from different manufacturer had different amounts and content.The total content of beer biogenic amine was 4.21-10.59 mg/L.It was too low to harm human health.Then this paper studied the relationship between the original wort concentration,alcohol content and the biogenic amine content.The results indicated that the two factors had some relationship with amine content,though they were relatively minor factors compared with different manufacturers and fermentation conditions.