草苁蓉水萃取物对四氯化碳致肝损伤小鼠肝脏氧化应激的干预作用

Intervention effect of aqueous fractions from Boschniakia rossica on hepatic oxidative stress in mice with liver injury induced by carbon tetrachloride

目的:研究草苁蓉水萃取物(BRAF)对四氯化碳(CCl4)诱发的急性肝损伤小鼠肝脏氧化应激的干预作用。方法:将实验小鼠随机分为对照组、模型组、水飞蓟素阳性对照组(50 mg.kg-1)及BRAF高、低剂量组(200,100 mg.kg-1)。每日给小鼠灌胃水飞蓟素或BRAF 1次,共7 d。实验末期,腹腔注射CCl4建立小鼠急性肝损伤模型,苏木素-伊红(HE)染色法观察肝组织病理学变化,比色法检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)活性及肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、Na+-K+-ATP酶(Na+-K+-ATPase)、Ca2+-Mg2+-ATP酶(Ca2+-Mg2+-ATPase)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)含量。结果:BRAF明显降低CCl4致急性肝损伤小鼠血清ALT,AST和ALP水平,减轻肝组织损伤,升高肝脏SOD,CAT,GPx和GSH水平,升高肝线粒体SOD,Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性,降低肝匀浆及线粒体MDA含量。结论:BRAF降低CCl4致急性肝损伤小鼠肝脏氧化应激,对CCl4致小鼠急性肝损伤具有保护作用。

Objective： To investigate the intervention effect of aqueous fractions from Boschniakia rossica （BRAF） on hepatic oxidative stress in mice with liver injury induced by carbon tetrachloride （ CC14 ）. Method： The experimental mice were randomly assigned into the normal control group, the model group, the silymarin （positive control） group, as well as high and low dose BRAF groups. Mice were treated intragastrically with silymarin or BRAF once every day for 7 days. At the end of the experiment, CC14 was injected intraperitoneally into the mice to establish the acute liver injury model. The pathological changes was detected with hematoxylin ＆ eosin （HE） staining, and the activities of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, alkaline phospha- tase （ALP）, superoxide dismutase （SOD）, catalase （CAT）, glntathione peroxidase （GPx）, Na＋ -K＋ -ATPase, Ca2＋ -Mg2＋ -ATPase, and the contents of reduced glntathione （GSH） and malondialdehyde （MDA） were detected by the colorimetric method. Result： BRAF significantly reduced ALT, AST and ALP activities in serum, alleviated hepatic injury induced by CC14, increased SOD, CAT, GPx and GSH levels in liver, and SOD, Na＋ -K＋ -ATPase and Ca2＋ -Mg2＋ -ATPase activities in liver mitochondria, and decreased the MDA content in liver and liver mitochondria. Conclusion： BRAF reduces hepatic oxidative stress in mice with acute liver injury in- duced by CC14, thereby showing the protective effect on mice with acute liver injury induced by CC14.