川崎病患儿血小板膜糖蛋白Ibα基因多态性分析

Investigation of platelet membrane glycoprotein Ibα gene polymorphisms in children with Kawasaki disease

目的研究血小板膜糖蛋白Ibα（GPIbot）基因HPA-2a／b多态性与KD发病及并发冠状动脉损伤（CAL）之间的关系。方法应用聚合酶链反应-限制性内切酶片断长度多态性分析技术结合琼脂糖凝胶电泳技术，检测30例KD患儿和60例健康对照组儿童GPIbα基因HPA-2a／b多态性位点的基因型和等位基因分布。结果本组KD病例和健康对照组儿童GPIbα基因HPA-2a／b的基因型均只有TC型和CC型，而未发现TT型。KD组GPIbα基因HPA-2a／b多态性的CC、TC、TT基因型分布频率和C、T等位基因频率与健康对照组比较差异均无统计学意义（χ2=0．052、0．048，P均〉0．05），KD组中合并CAL组与无CAL组基因型分布频率和等位基因频率比较差异亦无统计学意义（χ2=2．672、2．481，P均〉0．05）。结论GPIba基因的HPA-2a／b多态性与KD及其CAL的发生均无明显相关性。

Objective To investigate the association between platelet glycoprotein Iba （GPIbα） gene HPA- 2a/b polymorphisms and the risk of Kawasaki disease （KD） and that complicated with coronary artery lesion （CAL）. Methods A total of 30 patients with KD and 60 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis for the HPA-2a/b polymorphism in GPIbα gene. Resuits For HPA-2a/b polymorphism in GPIbα gene, there was only genotype TC and CC of HPA-2a/b polymorphism in GPlbol gene in children with KD and healthy controls, and genotype TT was not found in both groups. There were no significant differences between KD patients and the controls in genotype frequencies of CC, TC and TT and allele frequencies of C and T （χ2 = 0. 052,0. 048 ,all P 〉 0.05 ） ; also there was no significant difference between KD patients with CAL and that without CAL in genotype and allele frequencies （χ2 = 2. 672,2. 481, all P 〉 0.05 ）. Conclusion No association is found between HPA-2a/b polymorphism in GPIbα gene and the risk of KD or its complication of CAL in this study.