基质金属蛋白酶-9表达与早产胎膜早破的相关性研究

Study on correlation between between matrix metalloproteinase-9 expression and preterm premature rupture of membranes

目的:研究基质金属蛋白酶-9(MMP-9)在胎膜组织上的表达情况与早产胎膜早破的相关性。方法:荧光定量PCR(SYBR Green法)检测MMP-9mRNA;Western blot法检测MMP-9蛋白表达量;结果:早产胎膜早破组与足月胎膜早破组相比较,MMP9不存在统计学差异(Z=-1.783,P=0.075);与自发性早产组比较,MMP9不存在统计学差异(Z=-0.157,P=0.875);而早产胎膜早破组与正常足月产组比较MMP9存在统计学差异(Z=-3.316,P=0.001)。早产胎膜早破组MMP-9蛋白表达高于足月产组,之间的差异具有统计学意义(t=-12.339,P<0.01)。结论:MMP-9与早产胎膜早破存在相关性,在其发病机制中有着重要作用。

Objective： To study the expression of matrix metalloproteinase - 9 （ MMP - 9 ） in fetal membrane and its correlation with preterm premature rupture of membranes （PPROM） . Methods： Fluorescence quantitative PCR （SYBR Green method） was used to detect MMP- 9mRNA; Western blot was used to detect MMP- 9 protein expression. Results： There was no statistically significant differ- ence in MMP - 9 expression between PPROM group and term premature rupture of membranes （TPROM） group （ Z = - 1. 783, P = O. 075） ; there was no statistically significant difference in MMP - 9 expression between PPROM group and spontaneous premature delivery group （Z = -0. 157, P = 0. 875 ）; there was statistically significant difference in MMP- 9 expression between PPROM group and nounal term delivery group （Z = -3. 316, P =0. 001 ） . The expression level of MMP -9 protein in PPROM group was statistically significantly higher than that in TPROM group （t = - 12. 339, P 〈0. 01 ） . Conclusion： There is a correlation between MMP -9 and PPROM, MMP - 9 plays an important role in pathogenesis of PPROM.