UPLC法测定人血清中雷公藤甲素浓度

Determination of Triptolide Concentration in Human Serum by UPLC

目的:建立测定人血清中雷公藤甲素浓度的方法。方法:采用超高效液相色谱法,内标为艾司唑仑。色谱柱为Waters Acquity C18柱,流动相为乙腈-水(30:70,V/V),流速为0.2ml/min,柱温为室温,检测波长为220nm,进样量为5μl。结果:雷公藤甲素血药浓度在13.13～840.00ng/ml范围内同雷公藤甲素与内标物峰面积比值线性关系良好;日内、日间RSD均<15%,方法回收率为88.25%～99.33%。结论:该方法快速、灵敏、准确,可用于雷公藤甲素的血药浓度监测和药动学研究。

OBJECTIVE： To establish a method for determining the concentration of triptolide in human serum. METHODS： UPLC method was adopted. The column was Waters Acquit）, C18 column with mobile phase consisted of acetonitrile-water （30：70, V/V） at flow rate of 0.2 ml/min using estazolam as internal standard. The temperature of column was set at room temperature and the detection wavelength was set at 220 rim. The injection volume was 5 p.1. RESULTS： The linear ranges of triptolide were 13.13-840,00 ng/ml, and RSD of intra-day and inter-day was lower than 15%. The recoveries were 88.25%-99.33%. CONCLUSION： This method is rapid, sensitive and accurate, and can be used for pharmacokinetic study and serum concentration monitoring, of trintolide.