高效毛细管电泳法研究美洛培南和亚胺培南/西司他丁在腹膜透析液中的稳定性

STUDIES ON STABILITY OF MEROPENEM AND IMIPENEM/CILASTAIN IN PERITONEAL DIALYSIS SOLUTIONS BY HPCE

目的 :用高效毛细管电泳法研究美洛培南和亚胺培南 /西司他丁在腹膜透析液中的稳定性。方法 :运行缓冲液为 0 0 3mol·L 1磷酸硼砂缓冲液和 0 0 5mol·L 1的十二烷基硫酸钠 ,pH 8 5 ;操作电压 2 0kV ,检测波长 2 0 0nm。结果 :美洛培南、亚胺培南、西司他丁的线性范围为 10 0～ 10 0 0 μg·ml 1。结论 :美洛培南在腹膜透析液中不稳定 ,5℃放置 5小时后含量下降到 90 %以下。亚胺培南较美洛培南在腹膜透析液中稳定 ,在 5℃时可放置 14小时。西司他丁 5℃放置 14小时含量只下降 6 %。pH变化在 0 4以内。

OBJECTIVE:An HPCE method was applied to study the stability of meropenem and imipenem/cilastain in peritoneal dialysis solutions(PDS).METHODS:The measurements were carried out in a buffer,0.03 mol·L 1 phosphate borate and 0.05 mol·L 1 dodecyl sulfate,pH8.5,running voltage 20 kV,detection wavelength 200 nm.RESUITS:The linear Ca libration ranges were 100～1000 μg·ml 1 for meropenem,imipenem and cilastain.CONCLUSION:Meropenem was unstable in PDS stored at 5 ℃,20 ℃,37 ℃,its concentration decreased to less than 90% of the initial concentration within 5 hours at 5 ℃.Imipenem was more stable in PDS than meropenem.At 5 ℃ the stored duration of imipenem would be 14 hours.The concentration of cilastain decreased to 94% of the initial concentration in PDS stored at 5 ℃.The variation ranges of pH values for the PDS were ±0.4