牦牛病毒性腹泻病毒全基因测序及生物信息学分析

Genome Sequencing and Bioinformatics Analysis of Bovine Viral Diarrhea Virus Strain Yak

从牦牛(Yak)体内分离出牛病毒性腹泻/黏膜病病毒(BVDV/MDV),参考GenBank中已收录的BVDV-Ⅰ型的全基因组序列,设计了19对引物,通过RT-PCR方法,对牛病毒性腹泻病毒牦牛株进行克隆及测序,得到其全基因组序列(Gen-Bank登录号:JQ799141),序列全长12214nt,其中5′-UTR长288nt,3′-UTR长232nt。将牦牛株BVDV全基因序列与Gen-Bank中登录的其他8株BVDV病毒全基因序列进行同源性比对及系统进化分析,结果表明,牦牛株BVDV与BVDV-Ⅰ型毒株出于同一分支,但与其他8株BVDV毒株全基因序列同源性均不高,有可能属于新的独立基因型或基因亚型,仍需进一步研究证实。

In this study,the bovine viral diarrhea virus（BVDV） strain Yak was used as material to sequence the complete genome.Nineteen pairs of primers were designed according to the complete genome sequence of BVDV-Ⅰ virus,the whole genome of BVDV strain Yak was cloned and sequenced.Sequence analysis showed that the genome was 12214 nucleotides（nt） in length,which contained a 288 nt 5′-untranslated region（UTR） and a 232 nt 3′-UTR.The complete genomic sequence of BVDV strain Yak had already submitted into GenBank（GenBank accession NO： JQ799141）.Homologous analysis showed that homology of the whole genome sequence was low between BVDV strain Yak whole genome sequence and other 8 strains of BVDV whole genome sequences.Evolutionary tree results showed that the genetic evolution relationship was farther between BVDV strain Yak and other 8 strains of BVDV.