免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布

Distribution and Localization of Luteinizing-hormone Receptors on the Ovaries between Duolang Sheep and Karakul Sheep

为从形态学角度理解内分泌调节过程,揭示促黄体生成素(luteotropic hormone,LH)对雌性哺乳动物卵巢调节作用机制,试验采用免疫组织化学SP方法研究促黄体生成素受体(luteotropic hormone receptors,LHR)在多浪羊和卡拉库尔羊卵巢组织中的分布与表达,分别选取相邻的5张连续切片,光镜观察、图像分析。结果显示,多浪羊与卡拉库尔羊的LHR阳性物质主要见于膜细胞、卵母细胞、颗粒细胞和血管周围间质,尤其在膜细胞和卵泡颗粒细胞的胞质中分布最多。原始卵泡卵母细胞中便有LHR受体阳性物质分布,在各级卵泡中LHR阳性细胞数量和染色强度随卵泡发育呈正向增加趋势。

In order to understand the endocrine regulation process in a morphological view and reveal luteotropic hormone（LH） mechanism action in the female mammalian ovary,the distribution and localization of luteotropic hormone receptors（LHR） in ovaries of Duolang sheep and Karakul sheep were determined by immunohistochemical SP method.The results showed that 5 positive continuous sections were selected respectively.LHR-immunoreactive substance was mainly distributed over granulose cells,theca cells,oocyte,and ovary blood vessel surrounding interstitium.LHR was mainly loaded by the granulose cells and theca cells.Receptors regulation was showed during the earlier follicle period.With the development of follicles,the quantities and the staining intensity of the LHR-immunoreactive cells presented an increasing trend.