灵芝液体发酵产胞内多糖的培养基优化

Medium Optimization for Liquid Fermentation of Ganoderma lucidum Producing Intracellular Polysaccharides

以菌丝生物量及菌丝胞内多糖为指标,用液体发酵方法培养灵芝菌丝并获得菌丝胞内多糖,通过单因素及正交试验,研究了碳源、氮源、微量元素对灵芝菌丝生长及多糖产量的影响。结果表明:灵芝液体发酵生产菌丝多糖最适合培养基组合为:麦芽糖2.0%、酵母提取粉2.0%、ZnSO40.015%、VB10.10%、KH2PO40.2%、MgSO40.05%、pH 6.0,菌丝多糖产量高达6.64%。

The culture of Ganoderma lucidum mycelium was researched for production of the mycelial intracellular polysaccharides through liquid fermentation method, using mycelial biomass and mycelial intracellular polysaccharide as the indicator. And the influence of carbon sources, nitrogen sources and trace elements on the growth of Ganoderma lucidum mycelium and polysaccharide yield were evaluated through single factor and orthogonal experiment. The result showed that the perfect culture medium composition of producing mycelium with Ganoderma lucidum mycelium liquid fermentation was maltose 2.0%, yeast extract 2.0%, ZnSO4 0.015%, VB1 0.10%, KH2PO4 0.2%, MgSO4 0.05% and pH 6.0. Under the optimum conditions, the mycelial polysaccharide yield was up to 6.64%.