三种杀虫剂亚致死浓度对川硬皮肿腿蜂繁殖和搜寻行为的影响

Sublethal effects of three insecticides on the reproduction and host searching behaviors of Sclerodermus sichuanensis Xiao(Hymenoptera:Bethytidae)

在实验室条件下通过药膜法研究了3种杀虫剂对川硬皮肿腿蜂Sclerodermus sichuanensis Xiao成蜂的毒力和对繁殖的亚致死效应,并采用EthoVision3.1行为仪分析了杀虫剂对其搜寻行为的影响。结果表明,川硬皮肿腿蜂对啶虫脒最敏感,致死中浓度LC50和亚致死浓度LC10分别为7.71和4.46 mg/L,其次为吡虫啉(11.22和6.68 mg/L)和功夫菊酯(27.72和9.36 mg/L)。经啶虫脒、吡虫啉和功夫菊酯亚致死浓度(LC10)处理的肿腿蜂,亲代、F1和F2代的寄生成功率均受到抑制;经功夫菊酯处理的肿腿蜂,亲代单蜂产卵量显著增加(P<0.05);经啶虫脒和吡虫啉肿腿蜂,亲代的出蜂率没有显著影响(P>0.05),但对F1代影响显著(P<0.05)。经啶虫脒和吡虫啉亚致死浓度(LC10)处理的肿腿蜂搜寻行为变弱。结果表明,功夫菊酯对川硬皮肿腿蜂较安全;啶虫脒和吡虫啉对川硬皮肿腿蜂的繁殖和搜寻行为影响较大。研究结果对评估3种药剂对川硬皮肿腿蜂的安全性,为指导合理用药,协调化学防治和生物防治提供科学依据。

The toxicity of three kinds of insecticides on the adults of Sclerodermus sichuanensis Xiao and the sublethal effects of insecticides on their reproduction were studied under laboratory conditions, and video tracking capture system （EthoVision 3. 1 ） was employed to study the search behavior of S. sichuanensis adults. The results showed that Sclerodermus sichuanensis Xiao is the most susceptible one to acetamiprid. Lethal concentration of LCs0 and sublethal concentration （LC10） values were 7.71 mg/L and 4.46 mg/L respectively, and it is susceptible to imidacloprid （ 11.22 and 6.68 rag/L） and cyhalothrin （27.72 and 9.36 mg/L） in the second place. After treated by sublethal concentration of LC10 with acetamiprid, imidacloprid and cyhalothrin, the rate of successful parasitism for parental generation, F1 and F2 generation of S. sichuanensis was restrained. After treated with cyhalothrin, the amount of single fecundity of the parental was increased （P 〈 0.05 ）. After treated with acetamiprid and imidacloprid, there was no significant effect on the rate of reproduction of the parental （ P 〉 0.05 ）, but the F2 generation was greatly affected （ P 〈 0.05 ）. After treated by sublethal concentration of LC10 with acetamiprid, imidacloprid, the search behavior of S. sichuanensis were decreased. The results suggest that cyhalothrin is safe to S. sichuanensis. Acetamiprid and imidacloprid have a big effect on the reproduction and search behavior of S. sichuanensis. The results of this study can provide a scientific basis for safety evaluation of three kinds of insecticides on S. sichuanensis, rational use of insecticides and the coordination between chemical and biological prevention and control.