RNA沉默PI3K、AKT基因对人卵巢癌SKOV3细胞增殖和凋亡的比较

Comparison of RNA interference targeting PI3K and AKT on proliferation and apoptosis of ovarian cancer SKOV3 cells

目的探讨RNA技术分别沉默PI3K、AKT基因表达对人卵巢癌SKOV3细胞增殖与凋亡的比较,从而为卵巢癌的基因治疗提供有效的理论依据。方法体外合成PI3Kp110α、AKT序列特异性双链RNA(dsRNA),用lipofectamin2000转染人卵巢癌SKOV3细胞,采用MTT法检测细胞活性,流式细胞仪法检测细胞凋亡率。结果 MTT法检测siRNA-AKT组细胞增殖能力降低大于siRNA-PI3K组,差异有统计学意义(P<0.05),而流式细胞术检测siRNA-AKT组细胞凋亡率则高于siRNA-PI3K组,差异有统计学意义(P<0.05)。结论体外合成靶向AKT的siRNA更能有效地抑制人卵巢癌SKOV3细胞增殖及诱导细胞凋亡。PI3K通路并非AKT激活的唯一途径。

Objective To investigate the comparison of RNA interference targeting PI3K p110α or AKT on proliferation and apoptosis of ovarian cancer SKOV3 cells, which will offer an effective theoretical basis of ovarian cancer. Methods SKOV3 cells were transfected with PI3K pll0a or AKT sequence-specific dsRNA by lipofectamin2000. The cell activity was detected by MTT, and the apoptosis was tested by flow cytometry. Results Cell proliferation of siRNA- AKT group reduced than in siRNA-PI3K group（P〈0.05）, and apoptosis rate of siRNA-AKT group increased than in siRNA-PI3K group by flow cytometry（P〈0.05）. Conclusion AKT- gene-targeted siRNA could lead to the suppression of proliferation, and induce apoptosis of SKOV3 cells. PI3K pathway is not the only way of AKT activation.