摘要
目的:建立HPLC法同时测定感冒清热颗粒中葛根素与胡薄荷酮含量的方法。方法:采用DiamonsilC18色谱柱(250mm×4.6mm,5μm),用甲醇.水梯度洗脱,检测波长252nm,柱温40℃,流速1.0mL·min^-1,进样量10此。结果:葛根素、胡薄荷酮与其他杂质峰分离良好。葛根素在5.532~88.512gg·mL^-1。与峰面积呈良好的线性关系(r=1.0000);胡薄荷酮在0.786~12.573μg·mL^-1与峰面积呈良好的线性关系(r=0.9999),葛根素、胡薄荷酮平均加样回收率分别为100.73%和99.78%,RSD分别为0.62%(n=6)和0.75%(n=6);不同厂家生产的感冒清热颗粒中葛根素和胡薄荷酮的含量差异较大。结论:该方法操作简便.结果准确.可作为感冒清热颗粒的质量控制方法。
Objective: To establish a method for the simultaneous quantification of puerarin and pulegone in ganmao qingre granules. Methods: Chromatography was performed using a Diamonsil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol and water. The detection wave lengths was 252 nm and column temperature was 40 %. Results: The calibration curves of puerarin and pulegone were linear in the range of 5.532 - 88.512 μg·mL^-1 (r = 1.000 0) and 0.786 - 12.573 μg·mL^-1(r = 0.9999) respectively. The average recoveries were 100.73% and 99.78%; the values of RSD were 0.62%( n = 6 )and 0.75% (n = 6). Contents of puerarian in ganmao qingre granules from different manufacturers were different. Conclusion: The method is sensitive, accurate and can be used for the quality control ofganmao qingre granules.
出处
《中国药物应用与监测》
CAS
2013年第1期17-20,共4页
Chinese Journal of Drug Application and Monitoring