HPLC法同时测定感冒清热颗粒中葛根素与胡薄荷酮的含量

Content determination of puerarin and pulegone in ganmao qingre granules by HPLC

目的：建立HPLC法同时测定感冒清热颗粒中葛根素与胡薄荷酮含量的方法。方法：采用DiamonsilC18色谱柱（250mm×4．6mm，5μm），用甲醇．水梯度洗脱，检测波长252nm，柱温40℃，流速1．0mL·min^-1，进样量10此。结果：葛根素、胡薄荷酮与其他杂质峰分离良好。葛根素在5．532～88．512gg·mL^-1。与峰面积呈良好的线性关系（r=1．0000）；胡薄荷酮在0．786～12．573μg·mL^-1与峰面积呈良好的线性关系（r=0．9999），葛根素、胡薄荷酮平均加样回收率分别为100．73％和99．78％，RSD分别为0．62％（n=6）和0．75％（n=6）；不同厂家生产的感冒清热颗粒中葛根素和胡薄荷酮的含量差异较大。结论：该方法操作简便．结果准确．可作为感冒清热颗粒的质量控制方法。

Objective： To establish a method for the simultaneous quantification of puerarin and pulegone in ganmao qingre granules. Methods： Chromatography was performed using a Diamonsil C18 column （250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of methanol and water. The detection wave lengths was 252 nm and column temperature was 40 %. Results： The calibration curves of puerarin and pulegone were linear in the range of 5.532 - 88.512 μg·mL^-1 （r = 1.000 0） and 0.786 - 12.573 μg·mL^-1（r = 0.9999） respectively. The average recoveries were 100.73% and 99.78%; the values of RSD were 0.62%（ n = 6 ）and 0.75% （n = 6）. Contents of puerarian in ganmao qingre granules from different manufacturers were different. Conclusion： The method is sensitive, accurate and can be used for the quality control ofganmao qingre granules.