BARF1基因转染对人胃上皮细胞Bcl-2表达的影响

Effect of Bcl-2 expression for BARF1 gene transfected into human gastric epithelial cells

①目的探讨EB病毒编码BARF1基因对人胃上皮细胞Bcl-2表达的影响。②方法用携带BARF1基因的真核载体PIRES2-EGFP/BARF1,转染人胃上皮细胞系GES-1,G418筛选单克隆。用免疫组化法检测转染细胞及未转染细胞Bcl-2的表达状况。③结果获得稳定表达BARF1基因的人胃上皮细胞株,转染BARF1基因的细胞Bcl-2表达显著高于未转染和转染空载体GES-1细胞(P<0.01)。④结论 BARF1基因可能上调了人胃上皮细胞Bcl-2表达。

Objective To investigate effect of Bcl -2 expression for BARF1 gene transfected into human gastric epithelial cells. Methods BARFI gene carried by eukaryotic vector PIRES2 - EGFP/BARF1 was transfected into human gastric epithelial cell GES- 1, monoclonal was screened by G418. Bcl- 2 was detected by Immunohistocbemistry in each group（ including non- transfected GES - l ,transfected with empty vector GES - 1 and BARF1 gene transfected GES - 1 ）. Results The human gastric epithelial cells of stable expression BARF1 gene was obtained. The expression of Bcl - 2 was higher for the GES - 1 ceils of transfected BARF1 gene than those of untransfected GES - 1 and transfected with empty vector GES - 1. Conclusion The expression of Bcl - 2 may be regulated in human gastric epithelial cells of BARF1 gene transfected.