Toll样受体4、肿瘤坏死因子-α在溃疡性结肠炎大鼠结肠组织中的表达及意义

Expression and significance of toll-like receptor 4 and tumor necrosis factor-α in rat colon tissue with ulcerative colitis

目的:研究Toll样受体4(Toll-like receptor,TLR4)、肿瘤坏死因子-α(tumor necrosis facorα,TNF-α)在溃疡性结肠炎(ulcerative colitis,UC)大鼠模型结肠组织中的表达及相互关系,探讨TLR4信号通路在UC发病中的意义。方法:80只SD大鼠,随机抽取60只为模型组,另外20只为对照组。采用"三硝基苯磺酸+乙醇"法造模后,观察和评估结肠黏膜的大体形态和组织学变化。免疫组织化学方法检测TLR4、TNF-α在模型组与对照组的结肠组织中的表达水平。结果:造模成功率97%。TLR4、TNF-α在模型组结肠上皮和炎症细胞中的表达率分别是84.5%、89.7%,均高于对照组的20.0%、30.0%(P<0.01);TLR4、TNF-α的表达率随模型组的组织学分级增高而上升(P<0.01),且TLR4与TNF-α的表达水平呈正相关关系(P<0.01)。结论:UC大鼠结肠黏膜中TLR4、TNF-α的表达与UC的发生、发展密切相关。

Objective： To explore the expression of toll-like receptor 4 （TLR4） and tumor necrosis facor-α（TNF-ot） and analyse their relationships in rat colon tissue with ulcerative colitis （ UC ）, and investigate the significance of TLR4 signaling pathway in the pathogenesis of UC. Methods：Eighty SD rats were randomly divided into control group（ 20 rats） and model group （60 rats） which were made model using trinitrobenzene sulfonic acid and ethanol. The morphology and histological changes in the model group were observed. The expression of TLR4 and TNF-α in colonic mucosa in two groups were measured using immunohistoehemistry. Results： The successful rate of making model was 97%. The expression rates of TLR4 and TNF-α protein in UC colon epithelium cells and inflammatory cells were 84.5% and 89.7% respectively, which were higher than that of the control group of 30.0% and 20.0% （P 〈0.01 ）. The expression of TLR4 and TNF-α in colonic mucosa in the model group were significantly enhanced with histological grade increasing and its were a positive relationship（ P 〈 0.01 ）. Conclusions ：The expressions of TLR4 and TNF-α have some relation to the occurrence and development of UC.