雷公藤单体TW19雄性抗生育及免疫活性调节的研究

Studies on the Male Anti fertility and Immunological Effects of TW19

TW1 9系从雷公藤根皮中提取分离出的一个二萜类化合物 ,本文比较了 TW1 9对SD大鼠、ICR小鼠和昆明种小鼠的雄性抗生育作用 ,以及对 ICR小鼠免疫功能的影响。TW1 9连续 5周灌服给药 ,以交配试验观察抗生育效果。结果显示 TW1 9对 SD大鼠、ICR小鼠、昆明种小鼠抗生育作用的半数有效剂量 (ED50 )分别为 332 .0μg/kg、36 9.9μg/kg、2 86 .8μg/kg。TW1 9在抗生育有效剂量时能使昆明种小鼠及 SD大鼠附睾内精子数量和活动力下降 ,睾丸重量减轻 ,而对 ICR种小鼠没有影响。TW1 9对体外培养的 T、B淋巴细胞具有抑制作用 ,而抑制 ICR种小鼠体内抗体的生成 ,但是对于整体动物 B细胞的增殖和补体

TW19 is a diterpene compound isolated from root derma of Tripterygium Wilfordii Hook.F .. Its effects on immune function in ICR mice and male fertility in SD rat, ICR mice and Kunming mice were evaluated. TW19 was administrated by oral garage for 5 weeks. The anti fertility efficacy was assessed with mating tests. The results showed that ED 50 of anti fertility is 332.0 μg/kg/day, 369.9 μg/kg/day and 286.8 μg/kg/day respectively in SD rats, ICR mice and Kunming mice. Five weeks after TW19 treatment at anti fertility dose, number and motility of spermatozoa and testis weights reduced significantly in SD rats and Kunming mice, but no effects were observed in ICR mice. In ICR mice, TW19 inhabited splenic T and B cells proliferation in vitro and suppressed antibody formation in vivo .