TLCS测定附子大黄配伍前后大黄极性成分含量

Determination of Fuzi combined with Daihuang: polar components in Daihuang with TLCS method

目的采用TLCS测定大黄药材及其配伍后极性成分的含量。方法选择合适的展开系统和显色剂,以大黄酸为对照品,采用双波长锯齿扫描法,测定波长λS=432 nm,参比波长λR=700 nm,扫描速度20 mm/s。结果 TLCS法测定大黄极性成分以大黄酸计,A为0.270%,B为0.139%,C为0.138%,D为0.202%,E为0.229%,五者之和为0.977%;附子大黄配伍后对大黄中极性成分的影响呈上下波动,在比例为1:1时,极性成分相对较高;附子大黄配伍大黄后下的样品中,随大黄后下时间增加其极性成分均呈明显上升的趋势。结论TLCS法测定结果与紫外法测定的大黄结合蒽醌含量变化相似,提示以附子大黄1:1配伍时其极性成分最高,在大黄后下2 h大黄极性成分含量最高。

Objective To adopt TLCS determination of Daihuang and its compatibility in posterior polar components. Methods To choose an appropriate system and chromogenic agent, used Rhein as the control group, the dual wavelength zigzag scanning method, the detecting wavelength was kS = 432nm, the reference wavelength wask R = 70Ohm, scanning speed was 20mm＇ s. Results TLCS method for the determination of polar components in Daihuang$ polar components was Daihuang acid juice, A 0. 270 %, B O. 139 %, C 0. 138 %, D O. 202 %, E 0. 229 %, The sum of the five was O. 977 % ; After Fuzi combined with Daihuang, the Daihuang~ polar components fluctuated, when the proportion was 1 ： 1, the polar components of Daihuang was higher; the samples with time increased the polar component showed obvious upward trend. Conclusion TLCS determination and UV determination of Daihuang combined with anthraquinone have the similar changes, After Fuzi combined with Daihuang when the proportion was 1 ： 1, the polar components of Daihuang is highest, the polar component of Daihuang is highest after 2h.