静脉全血放置时间对网织红细胞参数结果的影响

Influence of storage time of venous blood samples on the results of reticulocyte parameters

目的探讨静脉全血标本的存放时间对网织红细胞(RET)检测结果的影响。方法用Sysmex XE-2100血液分析仪对贫血及非贫血患者静脉全血标本分别于采血后不同时间点连续检测,观察RET检测各项指标的变化。结果非贫血患者网织红细胞计数(RET#)及RET%的结果从采血后直至72 h差异均无统计学意义,而未成熟网织红细胞比率(IRF%)、高荧光强度网织红细胞(HFR%)、中荧光强度网织红细胞(MFR%)及低荧光强度网织红细胞(LFR%)的结果在72 h时才出现明显变化;贫血患者RET#及RET%的检测结果在72 h时出现明显降低(P<0.05),而IRF%、HFR%、MFR%及LFR%的结果从24h开始即出现明显变化。结论静脉全血采集后放置时间过长将影响RET检测结果的准确性,贫血患者应在采血后24h以内检测,方能较好反映结果的准确性。

Objective To investigate the influence of storage time of venous blood samples on the results of reticulocyte（RET） parameters. Methods The blood samples of patients with and without anemia were detected respectively at different time after bleeding by Sysmex XE-2100 hematology analyzer,and the changes of RET parameters were analyzed. Results In non-anemia patients,only immature reticulocyte（IRF%）,high fluorescence reticulocyte（HFR%）,middle fluorescence reticulocyte（MFR%） and low fluorescence reticulocyte（LFR%） had significant changes after bleeding 72h,while there was no statistical significance for reticulocyte count（RET#） and reticulocyte percentage（RET %）. However,in anemia patients,RET# and RET % reduced significantly after bleeding 72h（P〈0.05）,and IRF%,HFR%,MFR% and LFR% had significant changes after bleeding 24h. Conclusions Long time storage of venous blood samples has influence on the results of RET parameters. In anemia patients,the results can reflect the accuracy if the samples are detected within 24h after bleeding.