慢性上颌窦炎窦粘膜的免疫组化研究

Immunohistochemical study of the sinus mucosa during chronic maxillary sinusitis

目的 :探讨慢性鼻窦炎局部粘膜中免疫活性细胞和分泌免疫成分在防御炎症过程中的作用。方法 :采用免疫组化染色法对两型鼻窦炎粘膜的 9种免疫活性细胞和 4种分泌免疫成分进行检测。结果 :鼻窦炎 型中 T淋巴细胞、T4细胞亚群、B细胞、巨噬细胞明显高于正常对照组 (P <0 .0 1) ;鼻窦炎 型中 T淋巴细胞、T8细胞亚群、B细胞、肥大细胞、嗜酸性细胞明显高于正常对照组 (P<0 .0 1) ;两型中的 T4/ T8细胞比值有明显差异 (P<0 .0 1)。结论 :两型鼻窦炎局部粘膜某些免疫活性细胞的浸润存在一定差异 ,提示其发病机理及治疗方法的选择应有区别。

Objective:To study the protective role of some immunocompetent cells and some humoral immune factors in the local mucosa during chronic sinusitis.Method:Using immunohistochemical streptavidin biotin peroxidae complex(SABC) method to investigate the distribution and the numbers about the T lymphocytes and subsets,B cells,macrophages,NK cells,mast cells eosinophils,HLA DR antigen and IgA,IgG,complement C 3,lysozyme in the local mucosa of two type chronic sinusitis.Result:The numbers of T lymphocytes, T helper/inducer cells,B cells and macrophages in the mucosa of chronic sinusitis type 1 was significantly more than these in the healthy control (P< 0.01 ).The number of T lymphocytes, T suppressor cells,B cells, mast cells and eosinophils in the type 2 was significantly more than these in the healthy control (P< 0.01 ).The ratio of the helper cells to T suppressor cells significant different in the two type chronic sinusitis.Conclusion:The study showed that the infiltrating of immunocompetent cells was different between the two type chronic sinusitis.