蝙蝠葛酚性碱在大鼠小肠吸收特性研究

Study on Absorption Kinetics of Phenolic Alkaloids from Menispermum Dauricum in Rats' Intestines

目的研究蝙蝠葛酚性碱在大鼠小肠的吸收特性。方法采用大鼠肠灌流实验,主要从药物吸收部位、浓度和介质pH等三方面对蝙蝠葛酚性碱的吸收特性进行研究。结果蝙蝠葛酚性碱在大鼠小肠吸收速率(Ka)顺序为:回肠>空肠≈十二指肠;药物浓度对Ka无显著影响;蝙蝠葛酚性碱在pH 5.4,6.8,7.8介质的Ka差异有统计学意义(P<0.05)。结论蝙蝠葛酚性碱在大鼠小肠各肠段均有一定吸收,但在回肠段吸收更好;蝙蝠葛酚性碱的吸收机制为被动扩散;随着肠循环液pH增大,蝙蝠葛酚性碱Ka增大。

Objective To study the absorption of phenolic alkaloids from Menispermum Dauricum （PAMD） by rats＇ intestines. Methods Absorption kinetics was investigated by intestinal perfusion in vivo in rats. The effects of absorption site, pH value and drug concentration on PAMD absorption were studied. Results The absorption rate constant （Ka） of PAMD was ileum〉jejunum ≈ duodenum, in an order from highest to lowest. The concentration of PAMD had no significant effect on Ka. Ka was significantly different at pH value of 5.4, 6.8 and 7.8 , respectively （ P〈0.05 ）. Conclusion PAMD is absorbed at all segments of intestine in rats, but the best absorption occurs in ileum ; the absorption pathway of PAMD was passive diffusion, and the Ka of PAMD increases as the pH of intestinal solution increases.