桂郁金多糖抗凝血及纤溶活性研究

Anticoagulant and Fibrinolytic Activities of Polysaccharides from Curcuma kwangsiensis

目的研究桂郁金多糖体内抗凝血作用和含药血清体外纤溶活性。方法小鼠连续3 d灌胃给予高、中、低剂量桂郁金多糖,分别检测全凝血时间(CT)、凝血因子Ⅱ(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和凝血因子Ⅰ(Fbg),并将含药血清用纤维蛋白平板法进行体外纤溶试验。结果桂郁金多糖能明显延长CT、APTT和TT(P<0.05),对PT和Fbg无明显影响;含药血清体外无纤溶活性。结论桂郁金多糖具有显著的体内抗凝血活性,该药通过内源性凝血途径和共同凝血途径发挥抗凝血作用。

Objective To study anticoagulant and fibrinolytic activities of polysaccharides from Curcuma kwangsiensis. Methods The mice were administered by garage with low, medium and high concentrations of polysaccharides for 3 days. Clotting time （CT）, prothrombin time （PT） , activated partial thromboplastiu time （APTT）, thrombin time （TT） and fibrinogen （Fbg） of mice were detected. Fibrin plate method was performed to determine the fibrinolytic activ.iy of medicated serum in vitro. Results The CT, APTT and TT were prolonged significantly （ P〈0.05 ）, but the PT and Fbg did not change. ConClusion The polysaccharides from Curcuma kwangsiensis have anticoagulant activities and exert their anticoagulant activities through intrinsic pathway and common pathway of coagulation.