脐血浆中硫化氢与分娩宫缩发动关系的探讨

The relationship of hydrogen sulfide in cord plasma and the contraction of myometrium

目的:研究脐血浆中硫化氢(H2S)在分娩宫缩发动中的意义。方法:在新生儿娩出后采集脐带静脉血浆60例,根据分娩时有否发动宫缩分为两组,用敏感硫电极检测脐血浆中硫离子的浓度,并换算出H2S的含量;免疫组织化学方法检测胎盘中胱硫醚-γ-裂解酶(CSE)和胱硫醚-β-合成酶(CBS)的表达程度。结果:未发动宫缩组脐血浆中H2S含量高于发动宫缩组,差异有统计学意义(P=0.017)。免疫组化见CSE表达于胎盘绒毛的细胞滋养层胞浆中,未发动宫缩组的表达明显高于发动宫缩组,CBS在两组中均未见表达。结论:胎盘绒毛的细胞滋养层胞浆中含有CSE,不含CBS;脐血浆中H2S由细胞滋养层细胞参与调控生成;孕产妇脐血浆中H2S与宫缩发动有密切关系。

Objective：To explore the relationship between endogenous hydrogen sulfide （H2S）of the blood plasma and the contraction of myometrium. Methods：The sixty cord plasma samples were collected and divided into two groups according to the delivery has not launched the contractions. The concentration of H2S was assayed by sensitive sulphur electrode. The expressions of eystathionine beta- synthase （CBS） and eystathionine gamma-lyase （CSE） in placenta were measured by using immunohistoehemistry. Results： H2S contents in the group which mothers did not start uterine contraction were higher than that mother started uterine contraction （P--0.01）; The expressions of CSE were detected in cytoplasm of placental villi trophoblast ceils and at higher levels in the group with mothers did not start uterine contraction. The expressions of CBS had not seen in both two groups. Conclusion：Cytoplasm of placental villi tropho-blast cells are included CSE and no CBS. The production of H^S in cord plasma is regulated by trophoblast cells. H2S content in cord placenta is related to initiation of parturition.