电化学处理对小麦种子NaCl胁迫萌发生长的影响

Study on the Wheat Seeds Germination and Growth under NaCl Stress by Electrochemical Treatment

利用统计学方法分析了在NaCl胁迫下电化学处理后的小麦种子发芽势、发芽率,同时利用分光光度法测定了淀粉酶活性、过氧化物酶活性和丙二醛含量。结果表明:经过电化学方法处理的小麦种子在NaCl胁迫下其发芽率、发芽势和酶活性等生理指标均明显高于清水浸泡和Na2SO4溶液浸泡的小麦,而丙二醛含量则明显低于清水浸泡和Na2SO4溶液浸泡的小麦。其中,在相同浓度的NaCl胁迫下,相比于清水浸种和Na2SO4溶液浸种,电化学方法处理过的小麦种子的发芽势、发芽率、淀粉酶活性、过氧化物酶活性分别提高了6.00%～14.67%、4.66%～11.33%、8.95～11.42U、23.38～29.20U,而丙二醛含量则降低了0.45～1.07μmol/L。该结果表明,电化学方法对小麦种子萌发生长的抗NaCl能力有一定的促进作用。

The germination potential and the germination rate of wheat seed electrochemically treated under NaCl stress were analyzed by statistical methods. At the same time, the activities of amylase and peroxidase as well as the content of malonaldehyde were determined using spectro- photometry. The results show that the wheat seeds treated by electrochemical system under NaCl stress,compared with the ones only soaked with water or Na2SO4 solution, were significantly higher in physiological indexes such as germination potential, germination rate and enzyme activity but lower in the content of malonaldehyde. Comparing with the wheat seed soaked with water or Na2SO4 solution, the germination potential, the germination rate, the amylase activity and the peroxidase activity of wheat seeds treated by electrochemical system increased by 6.00% 14.67%, 4.66-11.33%,8.95-11.42 U and 23.38-29.20 U, respectively, under the same concentration NaCl stress while the content of malonaldehyde decreased by 0.45-1.07 μmol/L. As a conclusion, the resistance ability of the wheat seeds to NaCl solution was enhanced by the treatment with electrochemical system.