健脾疏肝活血化瘀法对人肝癌细胞裸鼠复种移植瘤的抑制作用观察

Observation of Inhibition Effects of Spleen Invigoration and Liver-qiDepression elief,Promotion of Blood Circulation by Removing Blood Stasis Methodon Infusion of HumanHepatocarcinoma Tumor-Causing Cells into Nude Mice

目的 :探讨健脾疏肝活血化瘀药物对人肝癌细胞复种移植瘤在裸鼠体内的抑瘤作用。方法 :建立肝癌(HepG2)小鼠模型 ,观察不同组方的健脾疏肝活血化瘀药物对其肿瘤的影响。结果 :Ⅳ号方 (黄芪、柴胡、莪术等 )与Ⅰ号方 (黄芪 )、Ⅱ号方 (柴胡 )、Ⅲ号方 (莪术 )相比,其肿瘤体积和体重以及抑瘤率的治疗后、治疗前后差值 ,均有显著性差异 ,P均<0.05。Ⅴ号方 (蒸馏水 )与Ⅰ、Ⅱ、Ⅲ、Ⅳ号方治疗后、治疗前后差值比较 ,亦有显著性差异。结论 :健脾疏肝活血化瘀药物有明显的抑瘤作用 ,且健脾疏肝活血化瘀法明显优于益气健脾法、疏肝理气法和活血化瘀法 ;从而 ,从治疗角度进一步反证了肝郁脾虚。

Objective: To explore the anti-tumor effect of spleen invigoration and liver-qi depression relief, promotion of blood circulation by removing blood stasis (IRPR) drugs on transplanted tumor in vivo of nude mice. Methods: The model of HepG2 hepatocarcinoma mice was produced and treated with different combinations of drugs for IRPR . The influence on tumors was observed. Results: The tumor volume,weight and anti-tumor rate after treatment, its differences before and after treatment in Group Ⅳ (Astragalus, Bupleurum, Zedoary, et al.), all had significant differences from GroupⅠ(Astragalus),Group II (Bupleurum),Group III (Zedoary),P<0.05. That of Group Ⅴ(Distilled water) also had significant differences from those of GroupsⅠ,Ⅱ,Ⅲ,Ⅳ. Conclusion: IRPR had obvious anti-tumor effects, moreover, the IRPR method was significantly superior to the method of spleen-invigorating qi-supplementation,the depressed liver relief method,the method of blood circulation promotion by removal of bloodstasis; thus the leading pathogenesis of primary carcinoma of the liver was thestagnation of the liver-qi and deficiency of the spleen,and accumulation of blood stasis was further disproved from the treatment angle.