5周亚低温状态游泳运动改善NAFLD大鼠的实验研究

The Experimental Study on the Amelioration of NAFLD Rats with 5-week Swimming Exercise in the State of Mild Hypothermia

目的:在NAFLD动物模型建造过程中,对其实施亚低温状态5周游泳运动联合干预,比对研究各组别鼠肝组织病理切片、血清脂肪代谢指标、鼠肝组织氧化应激指标和HSP70mRNA表达量及其HSP70生成量变化,旨在实验研究改善NAFLD的机制。方法:60只SD大鼠随机分为对照组(C)、高脂组(F)、常温游泳高脂组(TS)、常温浸泡高脂组(TI)、亚低温游泳高脂组(HS)和亚低温浸泡高脂组(HI),每组10只。建造NAFLD动物模型的过程中,给予相应高脂分组预定干预。5周后宰杀SD大鼠,分别制备鼠肝组织病理切片、测试相关指标和采用固相夹心法酶联免疫吸附实验(ELISA)检测肝脏组织HSP70含量、Real-TimePCR相对定量分析测试肝组织HSP70mRNA。结果:肝组织病理学切片HE染色显示:HS相比TS肝小叶结构更清晰,脂滴空泡视野数目显著性减少;HS组血清学脂肪代谢指标:TG、TC、FFA相比F组有非常显著性降低;LDL相比F组有显著性降低;HDL相比F组降低,但是没有显著性差异;HS组肝组织氧化应激指标:MDA相比F组有非常显著性降低;GSH、SOD相比F组有非常显著性升高;HS组的肝组织HSP70同对照组C、F组比较呈升高,有非常显著性差异(P<0.01);HS组的肝组织HSP70RNA Real-TimePCR表达量同对照组C、F组比较,均呈升高变化,有非常显著性差异(P<0.01)。结论:在SD大鼠NAFLD模型建造过程中,实施亚低温状态下联合有氧运动干预措施,可以诱导NAFLD肝细胞生成大量应激蛋白参与并加速脂质代谢、参与增强NAFLD肝细胞抗氧化应激能力的环节从而延缓NAFLD的发生与发展。

Objective： In the construction of NAFLD animal model,it conducts intervention in the rats in the mild hypothermia state.Methods： 60 SD rats were randomly divided into control group,high-fat group,high-fat group swimming at room temperature,high-fat group at room temperature immersion,mild hypothermia swimming high-fat group and the hypothermia-immersion high-fat group.Each group has ten rats.In the process of the construction of animal models of NAFLD,we give the corresponding high-fat groups intervention.SD rats were killed after 5 weeks,testing each group SD serum alanine triglyceride（TG）,total cholesterol（TC）,free fatty acids（FFA）,high-density lipoprotein（HDL）,low-density lipoprotein（LDL） and liver tissue MDA,SOD,GSH and HSP70,HSP70 mRNA Real-Time PCR.Results： From HE stained sections of liver histopathology,the SD rat animal model of NAFLD which mild hypothermia group swimmers compared to the which swim at room temperature under light microscope is that hepatic lobule structures are more clear and the number of lipid droplets in vacuoles vision significantly reduced.HS group serological indicators of fat metabolism： TG,TC,FFA compared with F group is very significantly lower and LDL significantly lower than F group and HDL lower than F group,but no significant difference.HS group of liver tissue oxidative stress indicators： MDA compared with F group is significantly lower;GSH and SOD compared with group F is very significantly increased.HS group of HSP70 in liver tissue compared with group C and F,there is a very significant increase.HS group of liver tissue HSP70 RNA Real-Time PCR expression compared with group C and F,there is a very significant increase.Conclusion： In the process of SD rats NAFLD model construction,the implementation of joint aerobic exercise intervention measures in mild hypothermia state can induce NAFLD liver cells generate a lot of stress protein involved in lipid metabolism,and enhance NAFLD liver cell antioxidant stress ability link in order to delay the occurrence and development of the NAFLD.