VIP对裸鼠MKN45移植瘤的影响

Effect of vasoactive intestinal polypeptide on MKN45 xenografts in nude mice

目的探讨血管活性肠肽(VIP)对裸鼠MKN45移植瘤的影响。方法将18只人胃癌MKN45细胞株的移植瘤裸鼠随机均分为三组,分别每天皮下注射VIP 10μg·100μl-1.只-1(VIP组)、VIP受体拮抗剂[D-p-C1-phe6,Leu17]-VIP(V4380)10μg·100μl-1.只-1(拮抗剂组)和PBS100μl/只(对照组)。每周称量裸鼠体重及移植瘤体积,4周后处死裸鼠,称量移植瘤重量及体积。用免疫组化及RT-PCR检测移植瘤组织中胞外信号调节激酶(ERK)蛋白及mRNA表达的变化。结果给药第28天,VIP组裸鼠体重明显轻于拮抗剂组(P<0.05)。给药的第14、21天,VIP组移植瘤体积明显小于其它两组(P<0.01)。VIP组ERK蛋白的表达显著低于拮抗剂组(P<0.05)。结论 VIP可能通过抑制肿瘤细胞ERK/NF-κB通路对人胃癌细胞MKN45裸鼠移植瘤有潜在抑制作用。

Objective To investigate the effect of vasoactive intestinal polypeptide （VIP） on MKN45 xenografts in nude mice. Methods When MKN45 xenografts had grown to approximately 30 in volume, 18 nude mice were equally randomized into three groups of A（treated with VIP 10μg·100μl^-1·mouce^-1 ） ,B（treated with VIP receptor antagonist 10μg·100μl^-1·mouce^-1 ） and C （treated with PBS 100μl/mouce）. The drugs were injected subcutaneously daily for 4 weeks. The volume of xenografts and body weight were measured weekly. The expressions of ERK protein and mRNA in xenografts were detected by immunocytochemistry and RT-PC1K Results On the 28th day after administration, body weight of nude mice in group VIP was less than that in group B（P〈0. 05）. On the 21st day after administration, the size of the xenografts in group A was larger than that in the other two groups（P〈0. 01）. The expression of ERK in xenografts in group A was lower than that in group B（P〈0. 05）. Conclusion VIP may potentially inhibit the growth of MKN45 xenografts in nude mice through its inhibitory effect on the ERK pathway.