RSV感染调节A549细胞NGF、miR-27a的表达

RSV infection regulates the expressions of NGF and miR-27a in A549 cells

目的探讨RSV感染后NGF及miR-27a的表达变化。方法将体外培养的肺泡Ⅱ型上皮细胞A549分为RSV感染组和对照组。应用RT-PCR法检测RSV感染后NGF mRNA及miR-27a的表达。结果 A549细胞感染RSV后产生合胞现象。与对照组相比,RSV感染能显著上调A549细胞NGF mRNA水平、下调miR-27a的表达(P<0.05)。结论 RSV感染后下调miR-27a表达,激活NGF,从而参与气道炎症的发生。

Objective To investigate the expressions of NGF and miR-27a in A549 cells after RSV infection. Methods A549 cells were incubated in vitro and divided into RSV infection group and control group. The expressions of NGF and miR-27a after RSV infection were detected by RT-PCR. Results Syncytial phenomenon was observed in A549 cells after RSV infection. Compared with control group, RSV infection significantly upregulated mRNA expression of NGF, but downregulated miR-27a expression. Conclusion RSV infection may play an essential role in airway inflammation via downregulating miR-27a expression and activating NGF in A549 cells.