胃癌组织芯片中EB病毒感染与核PTEN的关系

Association of Epstern-Barr virus infection and nuclear PTEN protein in gastric carcinoma tissue in tissues-micoarry

目的探讨胃癌组织中EB病毒(EBV)感染与抑癌基因PTEN的关系。方法选择胃癌芯片有效组织476例,用原位杂交法检测胃癌组织中EBV编码的小RNA(EBERs),分析EBV相关胃癌病理形态及临床特征;用免疫组化EnVision法检测核PTEN蛋白表达,分析EBV和核PTEN在胃癌的表达与患者淋巴结转移、Lauren′s分型的关系。结果 476例胃癌中检测出EBV感染18例(3.8%),其中的14例伴淋巴结转移癌,所有癌细胞均表达EBERs,EBV相关胃癌分化程度低。核PTEN在胃癌组织中的表达率为17.0%(81/476);核PTEN与EBV感染胃癌呈负相关。结论 EBV相关胃癌具有独特的临床病理特征,胃癌组织中的抑癌基因核PTEN对判断胃癌的恶性程度和估计预后有一定意义。

Objective To evaluate the association of Epstern-Barr virus （EBV） infection and nuclear PTEN protein in gastric carcinoma tissues in tissues-micoarry. Methods EBV infection was examined using EBV-encoded small RNAs（EBERs） in-situ hybridization. The expression of nuclear PTEN protein in gastric cancer tissues-microarry was detected by EnVision immunohistochemical method. The relationship between the expressions and lymph node metastasis, Lauren＇s classification was analyzed. Results Of 476 patients, 18（3. 8%） cases had intranuclear expression of EBV,of whom with EBV-associated gastric carcinoma, 14 patients were with regional lymph node metastasis, mostly were poor differentiated and expressed EBERs. The positive expression rate of nuclear PTEN protein in EBV-associated gastric carcinoma was 17. 0% （81/476）. Nuclear PTEN expression in EBV-associated gastric carcinoma was negatively correlated with EBV infection. Conclusion EBV-associated gastric carcinoma has the distinct clinicopathological features. Detection of nuclear PTEN in gastric carcinoma may have a certain value of evaluating the malignabcy and predicting the pregnosis.