玉米出籽率、籽粒深度和百粒重的QTL分析

QTL Analysis of Kernel Ratio, Kernel Depth, and 100-Kernel Weight in Maize (Zea mays L.)

为研究玉米出籽率、籽粒深度、百粒重的遗传机制,以豫82×沈137组配的229个F2:3家系为试验材料,采用复合区间作图法进行QTL定位分析。在3个环境下共检测到10个QTL。其中,控制出籽率、籽粒深度、百粒重相关QTL分别为3个、3个和4个,它们的联合贡献率分别为35.5%、28.1%和39.0%。位于第1染色体上介于标记umc1335与umc2236之间控制出籽率的QTLqKR1b和位于第9染色体上介于标记bnlg1209–umc2095之间控制百粒重QTLq100-KW9b,分别解释18.9%和11.7%的表型变异,且作用方式为加性效应,分析表明这些区域可能包含调控玉米籽粒性状关键基因,对剖析玉米产量形成机制具有重要的参考价值。

In order to study the genetic mechanism of kernel ratio （KR, %）, kernel depth （KD）, and 100-kernel weight （100-KW） in maize, we constructed a mapping population consisting of 229 F2：3 lines from the cross between inbred lines Yu 82 and Shen 137. QTL mapping and analysis for the three traits were conducted under the three environments by composite interval mapping （CIM） method. Three, three, and four QTLs were detected for kernel ratio, kernel depth, and 100-kernel weight, with the joint contribution rates of 35.5%, 28.1%, and 39.0%, respectively. One major QTL （qKRlb） controlling kernel ratio was detected on chromosome 1, locating in marker of interval umc1335-umc2236, explaining 18.9% of the phenotypic variation. Another major QTL qlOO-KW9b controlling 100-kernel weight was detected in marker of interval bnlg1209-umc2095 on chromosome 9, with explained 11.7% of the phenotypic variation, and gene action of additive effect. The results showed that some key genes for kernel characters are possibly contained in these regions, having an important research value to analyze the genetic mechanism of maize yield formation.