抗白粉病小麦近等基因系DNA甲基化的MSAP分析

DNA methylation analysis of near-isogenic lines (NILs) of wheat resistant to powdery mildew based on MSAP analysis

应用甲基化敏感扩增多态性(MSAP)技术检测小麦抗白粉病代换系6A/6V与感病品系京411的近等基因系NILs,及其亲本6A/6V和京411的基因组DNA甲基化变化.结果表明,NILs的整体甲基化水平(22.9%)略高于6A/6V(21.2%),低于京411(23.4%).分析其甲基化模式发现,NILs相对京411甲基化水平降低条带比例(2.89%)明显高于甲基化提高条带(0.55%),而相对抗病亲本6A/6V,NILs甲基化水平降低条带比例(2.96%)明显低于甲基化提高条带(4.20%),说明NILs的整体基因表达水平低于6A/6V,高于京411.

To investigate the DNA methylation pattern, the methylation-sensitive amplified polymorphism （MSAP） technique was used to detect the genomic DNA methylation changes of powdery mildew resistant substitution lines 6A/6V, susceptible strains of Jing 411 and its near isogenic lines （NILs）. The results showed that the total methylation level of NILs （22.9%） was slightly higher than 6A/6V （21.2%）, and the methylation level was lower than Jing 411 （23.4%）. Furthermore, the methylation pattern revealed that the percentage of demethylation bands（2.89%） in NILs was higher than that of hypermethylation bands （0.55%） in contrast with Jing 411, while the demethylation level （2.96%） in NILs was lower than hypermethylation level （4.20%） in contrast with the relative resistant parent 6A/6V. In summary, it can be con-cluded that the overall gene expression levels in NILs were lower than 6M6V and higher than Jing 411.