睾丸生殖细胞肿瘤及睾丸原位癌中鞘糖脂GB3的表达及意义

Expression of GB3 in testicular germ cell tumor and testicular carcinomain-situ

目的检测鞘糖脂神经酰胺三己糖苷(GB3)在睾丸生殖细胞肿瘤及睾丸原位癌组织中的表达,并探讨其临床意义。方法应用免疫组化方法检测62例睾丸生殖细胞肿瘤及14例睾丸原位癌组织标本中GB3的表达。结果 62例生殖细胞肿瘤中,54例GB3(+)(87%);14例睾丸原位癌中,11例GB3(+)(78.6%),其中8例强(+),其曲细精管内支持细胞及残留生精细胞均呈GB3(-)。结论睾丸原位癌是睾丸生殖细胞肿瘤早期阶段,睾丸生殖细胞肿瘤及睾丸原位癌细胞中鞘糖脂成分GB3改变,提示GB3在睾丸生殖细胞肿瘤发生发展过程中起着作用。GB3可作为睾丸生殖细胞肿瘤标记物,特别在睾丸原位癌早期诊断中有实用价值。

Objective To inverstigate the expression of GB3 and its significance in the testicular germ cell tumor （TGCT）, testicular carcinomain-situ（CIS）, and the preinvasive stage of TGCT. Methods 62 cases of TGCT and 14 cases of CIS were studied. The expression of GB3 was performed by try with anti-GB3 monoclonal antibody 38-13. Results The expression of GB3 was detected in 54 TGCL cases （87%）, and in 11 CIS cases （78. 6% ）, 27/54 TGCLs and 8/11 CISs, no reaction was seen in germ cells and Sertoli cells. Conclusion GB3 may play a role in TGCEs. It is also a tumour-associated marker of TGCT and especially useful for the detection of CIS lesion.