As_2O_3抑制人肝癌细胞侵袭及转移的实验研究

Study of As_2O_3 on invasion and metastasis of hepatoma cells in vivo

目的通过研究三氧化二砷(As2O3)对肝癌侵袭及转移基因的影响,探讨As2O3治疗肝癌的机制。方法利用人肝癌细胞系(Bel-7402)接种裸鼠,建立肝癌模型;实验分3组,测量各组裸鼠实体肿瘤的重量及体积,计算瘤重抑制率;检测AFP;肿瘤组织及双肺病理检查,计算肺转移结节;免疫组化法检测肿瘤组织的MIF、IL-8、bFGF及HIF-1α的表达情况。结果 As2O3治疗组平均瘤重和瘤体积较对照组明显减低,差异显著P<0.01;As2O3治疗组能够明显降低肺转移结节数目,并能降低MIF、IL-8、bFGF及HIF-1α的阳性表达,均有统计学意义(P<0.05)。结论 As2O3能抑制肝癌细胞的侵袭和转移,降低MIF、IL-8、bFGF及HIF-1α的表达。

Objective To study the effects of As203 on invasion and metastasis of hepatoma tumor model. Methods Hepatoma cell line （Be1-7402 cells）was established. After treated by different density of As2O3, the size and weighty of tumor were observed, inhibition ratio of tumor weight was calculated and AFP was detected. The pathology of tumor tissue and lung were examined. Pulmonary metastasis tubercle was counted. The expression of MIF, IL-8, bF- GF and HIF-1α were detected by immunohistochemical. Results The size and weighty of tumor were significantly decreased in As203 group compared with those in control group（P 〈 0.01 ）. The number of pulmonary metastasis tubercle and AFP after treatment of As203 were obviously lower than those of control group （P 〈 0. 01 ）. The As2O3 groups depressed expression of MIF, IL-8, bFGF and HIF-1α （P 〈 0.05 ）. Conclusion As2O3 can inhibit invasion and metastasis of hepatoma, further inhibit the expression of MIF, IL-8, bFGF and HIF-1α.