氯化胆碱对异丙肾上腺素诱导大鼠心肌成纤维细胞增殖及胶原合成的影响

Effect of choline chloride on isoproterenol-induced proliferation and collagen synthesis of rat cardiac fibroblasts

目的探讨氯化胆碱对异丙肾上腺素(Iso)诱导的心肌成纤维细胞(CFs)增殖和胶原合成的影响。方法体外培养新生Wistar大鼠心肌成纤维细胞,分为对照组、Iso组和Iso+氯化胆碱组。各组药物分别作用48 h。应用MTT实验方法检测心肌成纤维细胞增殖,羟脯氨酸试剂盒检测胶原合成。结果 10-4mol/L Iso明显促进CFs增殖(P<0.05),1 mmol/L氯化胆碱明显抑制10-4mol/L Iso诱导的成纤维细胞增殖(P<0.05)与胶原合成(P<0.05)。结论氯化胆碱抑制异丙肾上腺素诱导的心肌成纤维细胞增殖及胶原合成。

Objective To investigate the effect of choline chloride （choline） on isoproterenol （Iso）-induced cardiac fibroblasts （CFs） proliferation and collagen synthesis. Methods CFs were isolated from neonatal Wistar rats and cultured in vitro. They were divided into groups as follows ： control group, Iso group, Iso ＋ choline group. After 48 h, the proliferation of CFs was measured by MTT assay, the collagen synthesis was detected by hydroxyproline method. Re- suits CFs significantly increased by 10^-4 mol/L Iso（P 〈0.05） ,1 mmol/L choline obviously inhibited CFs proliferation and collagen synthesis （ P 〈 0.05 ）, which were mediated by 10^-4 mol/L Iso. Conclusion Choline chloride inhilaits the proliferation and collagen synthesis of CFs induced by Iso.