慢性心房纤颤犬心房组织中髓过氧物酶的变化

Changes of myeloperoxidase in atrial tissues of dogs with chronic atrial fibrillation

目的探讨慢性心房纤颤(房颤)犬心房组织中髓过氧物酶(MPO)的变化及其意义。方法健康杂种犬20条,随机分为房颤组(n=10)和对照组(n=10)。房颤组使用永久起搏器起搏24周,制备房颤犬模型;对照组不置入起搏器。取心房组织标本,分别采用ELISA法、同位素稀释气相色谱质谱法、Masson染色、明胶酶谱法测定MPO含量、3-氯酪氨酸含量、胶原容积分数(CVF)、基质金属蛋白酶-2和9(MMP-2、MMP-9)活性。结果房颤组心房肌组织MPO含量、3-氯酪氨酸含量、CVF、MMP-2和MMP-9活性较对照组显著升高(P<0.05)。MPO含量与CVF显著正相关(P<0.05)。结论 MPO可能是慢性房颤发生和维持的重要介质。

Objective To investigate the changes and significance of myeloperoxidase （MPO） in atrial tissues of dogs with chronic atrial fibrillation. Methods Twenty mongrel dogs were randomly divided into atrial fibr/llation group （n = 10） and control group （n = 10）. The High frequency pacing was performed in atrial fibrillation group with permanent pacemaker, but not in the control group. Twenty-four weeks after pacing, atrial tissue was taken. The contents of MPO content and 3-chlorotyrosine were measured by ELISA and stable isotope dilution liquid chromatography with on-line tandem mass spectrometry, respectively. The collagen volume fraction（CVF） and activities of matrix metalloproteinase-2 and 9 （ MMP2, MMP-9） were deter- mined by Masson staining and gelatin zymography, respectively. Results The levels of MPO, 3-chlorotyrosine, CVF and activities of MMP-2 and MMP-9 were significantly higher in atrial fi- brillation group than those in control group （P 〈 0.05 ）. The MPO content was significantly cor- related with CVF （P 〈 0.05 ）. Conclusion MPO is possibly one of important mediators mech- anistically involved in the initiation and perpetuation of chronic atrial fibrillation.