变形链球菌和远缘链球菌耐氟菌株粘附能力的体外研究

Binding Ability of Fluoride Resistant Strains of S.Mutans and S.Sobrinus in Vitro

目的 探讨变形链球菌和远缘链球菌耐氟突变后 ,其粘附能力的改变。方法 用同位素标记方法测定变形链球菌、远缘链球菌亲代及耐氟菌株粘附至唾液包被羟基磷灰石 (SHA)表面的粘附量及粘附百分率 ,并比较两者的粘附能力。结果 变形链球菌、远缘链球菌亲代菌株与耐氟菌株间粘附百分率无明显差异 (P >0 .0 5 ) ;远缘链球菌与变形链球菌相比 ,其粘附百分率偏低 ,差异有显著性 (P <0 .0 1)。结论 变形链球菌与远缘链球菌耐氟菌株粘附至SHA表面的能力与亲代野生株无明显区别 。

Objective To investigate the binding ability of S.mutans and S.sobrinus after their fluoride resistant mutation.Methods The binding amount and binding percentage of S.mutans, S.sobrinus and their F resistant strains to saliva coated hydroxyapatite(SHA)were measured and compared by 3H labeled binding assay.Results The binding percentages of F resistant and their parent strains had few difference( P >0.05),whereas the binding percentage of S.sobrinus was significantly lower than S.mutans( P <0.01). Conclusions The data showed that the binding ability to SHA between F resistant and F sensitive S.mutans had no significant difference which indicated that the emerge of F resistant strains could not reduce the cariogenicity of S.mutans and S.sobrinus.