牙囊细胞对炎症组织来源牙周膜干细胞增殖、成骨和干性作用的影响

Effects of dental follicle cells on the proliferation,osteogenesis and stemness of periodontitis tissue derived periodontal ligament stem cells

目的:研究牙囊细胞(Dental follicle cells,DFCs)对炎症组织来源的牙周膜干细胞(peri-odorntitis tissue derived periodontal ligament stem cells,PPDLSCs)增殖、成骨能力和干性作用的影响。方法:利用transwell小室进行DFCs与PPDLSCs共培养,通过克隆形成率和细胞周期实验对共培养的PPDLSCs增殖能力进行检测;成骨诱导实验观察其成骨分化能力;同时利用RT-PCR检测成骨相关基因和干性相关基因的表达水平。结果:和对照组相比,与DFCs共培养后的PPDLSCs克隆形成率增强(P<0.05),处于增殖期细胞增多;成骨诱导后矿化结节明显增多,成骨相关基因Runx2,OCN,ALP明显上调(P<0.05);干性相关基因Oct4,Sox2,Klf4的表达也大幅上调(P<0.05)。结论:与DFCs共培养可以增强PPDLSCs的增殖与成骨分化能力,并增强其干性基因的表达。

AIM： To study the effects of dental follicle cells （DFCs） on the proliferation, osteogenesis and stemness of periodontitis tissue derived periodontal ligament stem cells （PPDLSCs）. METHODS ： The PPDLSCs and DFCs were co-cultured in transwells with 0.4μm- intersaeptum between chambers. The proliferation, osteogenic dif-ferentiation and stemness of PPDLSCs were examined by colony forming assay and cell cycle analysis, osteogenic induc- tion experiment and RT-PCR, respectively. RESULTS ： Compared with controls, the colony forming rate of PPDLSCs co-cuhured with DFCs was higher, and the percentage of cells in G2 ＋ M phase was larger. Meanwhile, there were more mineralized nodules in the co-cultures. PPDLSCs co-cultured with DFCs expressed higher mRNA levels of Runx2, OCN, ALP, Oct4, Sox2 and Klf4. CONCLUSION： DFCs can promote the proliferation, osteogenesis and sternness of PPDLSCs.