厌氧菌代谢酸产物的气相色谱分析

An Analysis of Metablic Organic Acid of Anaerobes by Gas Chromatography

目的 探讨厌氧菌代谢有机酸色谱分析法的可靠性 ,为大量的临床分离的厌氧菌鉴定提供可靠方法。方法 利用气相色谱法对 6种厌氧菌的国际参考菌株的代谢有机酸进行了定性定量测定。结果 6 0株细菌中 ,有48株细菌测到所有代谢有机酸 ,10株细菌测到主要代谢有机酸 ,2株细菌测到不应有的代谢有机酸 ,总符合率为97 6 7%。

Objective The purpose of this study was to test the reliability of measurement of metablic organic acid of anaerobic bacteria by gas chromatography for the identification of anaerobes.Methods The metablic organic acid of 60 strains of 6 species of oral residential anaerobes were tested by gas chromatography qualitatively and quantitatively. Results It was found that the types and quantities of metablic organic acid of all anaerobes but two strains of lactobacillus were coincident with the standard of identification of anaerobes,and the total coincidence was 97.67%.Conclusion The anaerobes could be identificated accurately and quickly by gas chromatography.