力学刺激对成骨细胞ClC-3氯通道影响的实验研究

Effect of mechanical stimulation on ClC-3 chloride channel in osteoblasts

目的:观察不同时长的持续静压力刺激对成骨细胞形态和ClC-3氯通道的影响。方法:取小鼠成骨样细胞系MC3T3-E1随机分为两组,实验组在1atm持续性静压力下培养;对照组常规培养。培养8、24 h取各组细胞在倒置显微镜下观察细胞形态;q-RT PCR检测细胞Clcn3 mRNA表达水平。结果:持续静压力刺激后8 h,细胞形态与对照组相比无明显改变,但24 h后,细胞更细长,细胞间隙变大。q-RT PCR检测显示,压力刺激后各时间点成骨细胞Clcn3 mRNA表达水平均明显高于对照组(P<0.05),并且随压力刺激延长,Clcn3 mRNA表达水平也随之升高(P<0.05)。结论:一定时间和大小的力学刺激能引起成骨细胞形态及ClC-3氯通道的变化。

AIM： To observe the effects of persistent static pressure on the shape andClcn3 expression of mouse osteoblast cell line MC3T3-E1. METHODS： MC3T3-E1 cells were cultured under 1 arm persistant static pressure or without the pressure（ the controls）. 8 and 24 h after culture, cell morphology was observed by inverted mi-croscope. Clcn3 mRNA expression was detected by q-RT PCR method. RESULTS ： No significant change in cell mor-phology was found after 1 atm continuous stimulation for 8 h. However, cells became more elongated, and the gap be-tween cells was bigger after 24 h stimulation. Persistent static pressure showed a time-dependent stimulatory effects on Clcn3 mRNA expression （ P 〈 0.05 ）. CONCLUSION ： Mechanical stimulation can lead to changes in cell morphology and CIC-3 chloride channel mRNA expression in MC3T3-E1 cells.