肿瘤坏死因子基因多态性与男性青少年品行障碍的关联

Association between tumor necrosis factor gene polymorphism and conduct disorder in male adolescents

目的探讨青少年男性品行障碍(Conduct Disorder,CD)患者与肿瘤坏死因子(Tumor NecrosisFactor,TNF)基因多态性的关系。方法采用SNaPshot基因分型技术对72例CD青少年男性和178例正常男性进行TNF基因rs4698107和rs16891867位点的基因分型。分析CD组与对照组的等位基因和基因型分布规律。将CD组患者根据是否存在注意缺陷多动障碍(Attention Deficit Hyperactivity Disorder,ADHD)分两亚组,比较两亚组及对照组的等位基因和基因型分布。结果 CD组rs4698107位点A等位基因频率高于对照组(24.6%vs.16.0%,P=0.025),OR值为1.72,95%CI为(1.07,2.76),基因型分布在两组中存在统计学差异(P=0.018)。CD组分亚组后,ADHD组、非ADHD组和对照组rs4698107位点基因型分布存在统计学差异(P=0.012),其中ADHD组基因型分布与对照组比较,差异有统计学意义(P=0.003)。其余组间差异无统计学意义。结论 TNF基因多态性与青少年品行障碍存在相关性,其相互关系值得进一步探讨。

Objective To study the association between Tumor Necrosis Factor （TNF） related gene polymor- phisms with conduct disorder in male adolescents. Methods Two single nucleotide polymorphisms （TNF rs4698107 and rs16891867） were detected using SNaPshot genotyping assay in 72 conduct disorder male adolescents and 178 normal adult controls. The distribution of allelic and genotypic frequencies in the case and control groups were ana- lyzed. The Conduct Disorder adolescents are divided into two sub-groups according to whether accompanied with Attention Deficit Hyperactivity Disorder （ADHD） symptoms. The two sub-groups are then respectively compared with the control group about the distribution of allelic and genotypic frequencies. Results The frequency of rs4698107 A allele in conduct disorder male adolescents was higher than that in the control group （24.6% vs. 16.0%, P=0.025, OR=1.72, 95%C1=1.07-2.76）. The difference in genotype distribution was significant between the two groups （P=0.018）. After the conduct disorder adolescents divided into two sub-groups, there were significant differ- ences in genotype frequency of rs4698107 among the three groups （P=0.012）. Further analysis found that there is significant difference in genotype frequency in ADHD group compared with control group （P=0.003）.There were no significant differences between the other groups. Conclusions The variance of TNF gene polymorphism mayplay a role in conduct disorder in male adolescents, and should be further researched.