应用组织工程化皮肤修复皮肤全层缺损

Autologous Tissue Engineered Skin for Full Thickness Skin Replacement

目的 本研究旨在评价组织工程技术修复皮肤缺损的可行性。方法 采用Yorkshire猪 ,在其背部脊柱两侧做 6个直径 4cm的圆形全厚皮肤缺损 ,随机分为 3组。第 1组为空白对照 ;第 2组仅置无细胞的pluronic;第 3组分别用角朊细胞 pluronic、成纤维细胞 pluronic复合物置于创面上。第 4、6周时取材 ,进行大体、组织学和透射电镜观察。结果 4 6周时表皮和真皮间界面明显被上皮嵴和真皮乳头所分隔。对照组无皮肤形成 ,仅被新生炎性肉芽组织所替代。组织工程化皮肤边缘收缩 ,组织学显示与正常皮肤相似 ,可见表皮分层及其下较厚的真皮。透射电镜可见 ,基板发育良好 ,并与基质紧密相连。结论 以pluronicF 12

Objective To evaluate the feasibility of regenerating or repairing damaged skin utilizing tissue engineering techniques. Materials and Methods Yorkshire pigs were used in the study. A critical size defect of six, full thickness, 4 cm in diameter round wounds, which were marked on the paravertebral region of the animal were excised. The skin defects were randomly divided into three groups. Group I as control group received no cells or polymer. Group II as a second control group received pluronic hydrogel with no cells. Group III as the experimental group received a mixture of cells (keratinocytes and fibroblasts) and pluronic hydrogel. All specimens were harvested at 4 and 6 weeks in vivo, and underwent gross, histological, and transmission electron microscope evaluation. Results Histologically, the skin in the experimental group was similar to normal skin with stratified epidermis overlying a moderately thick collageneous dermis. The interface of the tissue was apparently demarcated by epidermal ridges and dermal papillae. The control groups showed no skin formation except for granulation with infiltrating inflammatory cells in the wound. Transmission electron microscopy showed that the basal lamina of the experimental group was well developed and attached to the extracellular matrix. Conclusion This finding demonstrates the successful use of tissue engineered skin with Pluronic F 127 as a cell carrier.