解淀粉芽孢杆菌芽孢漆酶在染料脱色中的应用

Application of spore laccase from Bacillus amyloliquefaciens in dye decolorization

解淀粉芽孢杆菌LC03的芽孢漆酶具有较好的稳定性,通过制备具有漆酶活性的芽孢悬液,研究了芽孢漆酶对4种合成染料RB亮蓝、活性黑、靛红和结晶紫的脱色效果,并筛选出促进染料脱色的漆酶介体,同时考察了酶浓度、介体浓度对模拟染料废水脱色的影响。结果表明:RB亮蓝﹑活性黑和靛红在无介体时不能被脱色,在乙酰丁香酮(ACE)介导下的脱色率都超过了60.00%;在pH9.0时,芽孢漆酶-介体系统对模拟染料废水脱色的酶浓度和介体浓度分别为88.64U/L和0.5mmol/L时,2h后脱色率可超过80.00%,表明该芽孢漆酶在染料废水的处理上具有较好的应用前景。

Spore laccase from Bacillus amyloliquefaciens LC03 has good stability. In this work, spore suspension with laccase activity was prepared and tested for its ability in decolorization of four synthetic dyes,including remazol brilliant blue R （RBBR） , reactive black 5, indigo carmine and crystal violet. Laccase mediators were screened to improve the decolorization process. The effects of enzyme and mediator concentration on simulated dye effluent decolorization were also investigated. The results showed that no decolorization of RBBR, reactive black 5 and indigo carmine occurred in the absence of mediator, while more than 60.00% decolorization of the three dyes were obtained when acetosyringone （ACE） was present. The spore laccase-mediator system could efficiently decolorize the simulated dye effluent with 88.64 U/L of spore laccase and 0.5 mmol/L of ACE at pH 9.0. More than 80.00% of simulated dye effluent was decolorized after 2 hours, indicating potential application of this spore laccase in dye wastewater treatment.