东北地区野生鸟类J亚群禽白血病分子流行病学调查及部分基因组序列分析

Molecular Epidemiology and Part Genome Sequence Analysis of Subgroup J Avian Leukosis Virus Isolated from Wild Birds in Northeast China

为了解野生鸟类感染禽白血病(AL)的情况,于2010—2011年采集300份野生鸟类样品进行试验。用PCR方法先后检测到7株ALV-J阳性样品。分别扩增了env基因和3′UTR区段,并对其进行了序列测定和比较分析。结果显示,7株ALV-J阳性样品的env基因遗传进化与原型毒株HPRS-103和代表株不在同一个分支上,在3′UTR区段没有太大差异,与原型毒株HPRS-103相比,E元件部分位点存在变异。以上结果表明,目前中国野生鸟类已存在禽白血病的感染,虽然检出率较低,但是由于野生鸟类活动范围比较大,给AL的防控增加了难度。通过对J亚群阳性样品进行序列分析和比对,发现env基因和3′UTR区段部分位点发生变异。这些都提醒注意防控白血病在野生鸟类中进一步传播。

In order to analyze the situation of wild birds infected avian leukosis virus（ALV） in China in 2010-2011, we collected 300 samples of wild birds from different areas. Seven strains of ALV-J were detected by PCR. The env gene and the 3r UTR were amplified, cloned and sequenced from seven isolated strains, which were subgroup J-positive. The results showed that the env genes of the seven strains from positive samples were not in the same branch with prototype strain HPRS-103 and representative strains, and the 3＇UTR of them were a little different from HPRS-103. Part sites of E element have mutation, compared with HPRS-103. ALV has infected the wild birds in China. The large circumscription of the wild birds increases the difficulty of prevention and control of AL, though the relevance ratio is low. Parts of the env gene and 31UTR of isolated strains have varied by sequencing and analyzing. More attention should be paid in preven- ting and controlling the transmission of ALV among wild birds.